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Localization of mRNA Coding for the Three Subtypes of Atrial Natriuretic Factor (ANF) Receptors in Rat Anterior Pituitary Gland Cells
Author(s) -
Grandclément Béatrice,
Brisson Christine,
Bayard Francis,
Tremblay Johanne,
Gossard Francis,
Morel Gerard
Publication year - 1995
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1111/j.1365-2826.1995.tb00739.x
Subject(s) - anterior pituitary , medicine , endocrinology , thyrotropic cell , corticotropic cell , prolactin cell , messenger rna , biology , receptor , pituitary gland , gonadotropic cell , in situ hybridization , somatotropic cell , hormone , gene , biochemistry
Atrial Natriuretic Factor (ANF) action is mediated by highly selective and specific receptors. Three subtypes have been characterized and cloned: ANF receptor‐A, ‐B and ‐C. These subtypes are all expressed in the anterior pituitary of the rat. In the present study, the mRNA for each subtype was detected by in situ hybridization. The amounts of ANFR‐A and ‐B mRNA were found to be similar, and to be twice that of ANFR‐C mRNA. At the ultrastructural level, the three types of ANFR mRNA were expressed in three anterior pituitary cell types, namely lactotrophs, corticotrophs, and gonadotrophs, identified by their hormonal content. No signal was revealed in somatotrophs or thyrotrophs. The different forms of mRNA were similar in terms of subcellular localization: in the cytoplasmic matrix and the nuclear euchromatin. These data indicate that the anterior pituitary is an important target tissue for ANF action.