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Colocalization of Natriuretic Peptide and Estrogen Receptor Immunoreactivities in Preoptic Nuclei in the Female Rat
Author(s) -
Watson Robert E.,
Hutchinson Robert K.,
Langub M. Chris,
Landis John W.,
Seksaria Shikha,
Rainey David M.,
Keil Lanny C.
Publication year - 1994
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1111/j.1365-2826.1994.tb00557.x
Subject(s) - colocalization , medicine , endocrinology , preoptic area , natriuretic peptide , estrogen , brain natriuretic peptide , receptor , biology , estrogen receptor , atrial natriuretic peptide , hypothalamus , neuroscience , heart failure , cancer , breast cancer
Estrogen is known to play an important role in regulating reproductive function in female rats through actions exerted at the preoptic area, a part of the brain that is markedly sexually dimorphic and which contains abundant estrogen receptors. A critical question to our understanding of estrogen's action on the brain is to identify the types of neurons that contain estrogen receptors (ER). Previous studies have shown that atrial natriuretic peptide (ANP) is in abundance in the preoptic area, and that ANP and other natriuretic peptides are capable of regulating gonadotropin secretion. In an effort to determine whether ERs are present in natriuretic peptide‐immunoreactive (NP‐ir) neurons in the preoptic area of the rat, double label immunocytochemistry was performed. Since ER‐ir, as demonstrated with antibody H222 is known to be localized predominantly in cell nuclei, while NP‐ir is present in the cytoplasm, single cells can be double labeled. Diaminobenzidine tetrahydrochloride was used for localization of NP‐ir neurons, while nickel‐enhanced diaminobenzidine tetrahydrochloride was used for localization of ER‐ir. The results revealed that many nuclei throughout the preoptic area contained neurons that were ER‐ir or NP‐ir and that a substantial number were double labeled. Cell counts in selected preoptic nuclei and components, including the anteroventral periventricular nucleus, periventricular preoptic nucleus, medial part of the medial preoptic nucleus, and central part of the medial preoptic nucleus revealed that 13.6%, 11.1%, 13.5%, and 24.4%, respectively, of the NP‐ir neurons in these nuclei also contained ER‐ir. Collectively, a total of 14.9% of the NP‐ir neurons in these nuclei also contained ER‐ir. These results support the hypothesis that estrogen may regulate the activity of NP‐ir cells in the preoptic area and thereby may be capable of altering functions associated with NPs in this region of the brain, including not only regulation of gonadotropin secretion, but also control of blood pressure and fluid balance.