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In vitro Regulation of Rat Prolactin Messenger Ribonucleic Acid Poly(A) Tail Length: Modulation by Bromocriptine
Author(s) -
Carter David A.,
LiJin Chew,
Murphy David
Publication year - 1993
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1111/j.1365-2826.1993.tb00382.x
Subject(s) - prolactin , medicine , endocrinology , bromocriptine , messenger rna , dopaminergic , biology , prolactin cell , gene expression , hormone , anterior pituitary , in vitro , endocrine system , pituitary gland , gene , dopamine , biochemistry
Recent analysis of endocrine gene transcripts has revealed that several hormone mRNAs exhibit regulated size changes (due to alterations in length of the 3’poly(A) tail) which may function as an additional level of control in the determination of gene expression. We have now shown, through the novel application of an organ culture technique, that prolactin mRNA exhibits a similar regulated change in poly(A) tail length when rat anterior pituitary glands are explanted. The effect is observed in glands of either male or female rats and is specific with respect to growth hormone and α‐tubulin mRNAs. Furthermore, we have also found that the size change in prolactin mRNA is attenuated in the presence of bromocriptine, indicating regulation through a dopaminergic pathway.