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Removal of 3’Untranslated Sequences Dramatically Enhances Transient Expression of Ovine Follicle‐Stimulating Hormone Beta Gene Messenger Ribonucleic Acid
Author(s) -
Mountford Peter S.,
Brandon Malcolm R.,
Adams Timothy E.
Publication year - 1992
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1111/j.1365-2826.1992.tb00215.x
Subject(s) - messenger rna , complementary dna , gene expression , follicle stimulating hormone , microbiology and biotechnology , biology , untranslated region , transfection , medicine , endocrinology , gene , hormone , luteinizing hormone , genetics
The influence of the 3’untranslated (3′‐UT) region of the ovine follicle‐stimulating hormone (FSH) β mRNA on the level of transcript expression was studied. Only very low levels of FSH β mRNA were detected following transient transfection of COS cells with a eukaryotic expression vector containing a full‐length ovine FSH β cDNA that includes 1.1 kilobases (kb) of 3′‐UT. In contrast, deletion of all but 135 basepairs (bp) of the 3′‐UT resulted in a striking increase in FSH β mRNA expression following transfection of the truncated cDNA construct. These observations suggest that sequences within the 3′‐UT of the ovine FSH β mRNA may play a significant role in the post‐transcriptional regulation of FSH β expression.