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Expression of Histamine H 1‐ Receptors in Xenopus Oocytes Injected with Messenger Ribonucleic Acid from Bovine Adrenal Medulla: Pertussis Toxin Insensitive Activation of Membrane Chloride Currents
Author(s) -
Meyerhof Wolfgang,
Schwärzt Jürgen R.,
Höllt Volker,
Richter Dietmar
Publication year - 1990
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1111/j.1365-2826.1990.tb00446.x
Subject(s) - pertussis toxin , histamine , cimetidine , medicine , endocrinology , histamine h2 receptor , receptor , histamine h1 receptor , extracellular , chemistry , biology , biochemistry , g protein , antagonist
Histamine H 2 ‐receptors have been identified in Xenopus oocytes previously microinjected with poly(A) + ribonucleic acid from bovine adrenal glands. Bath application of histamine to ribonucleic acid‐primed oocytes evoked concentration‐dependent, oscillating membrane currents under voltage‐clamp conditions. H 1 ‐receptor specific antagonists clemastine, doxepin, pyrilamine, promethacine, diphenylhydramine, dephenylpyraline and chlorpheniramine, but not H 2 ‐receptor antagonists, cimetidine and ranitidine, inhibited histamine‐induced responses. Membrane currents evoked by bath‐applied histamine were insensitive to pertussis toxin, carried by chloride ions and dependent on intracellular but not extracellular calcium.