Premium
Switching Between Two Types of Bursting Activity of Single Ca +2 ‐Activated K + Channels in Dissociated Neurons
Author(s) -
Yamamoto D.,
Pinnock R. D.,
Sattelle D. B.
Publication year - 1989
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1111/j.1365-2826.1989.tb00085.x
Subject(s) - bursting , gating , patch clamp , chemistry , membrane potential , biophysics , electrophysiology , physics , neuroscience , biology
Single channel recordings of Ca 2+ ‐activated K + currents were made from dissociated cockroach neurons by means of the gigaohm‐seal patch‐clamp technique. Bursts of single channel openings were composed of two distinct classes: the ‘long‐open burst’ contained groups of long, rectangular, pulse‐like openings with durations of 3.5 to 1.2 ms (depending on membrane potential), whereas the ‘flickering burst’ consisted of clusters of brief openings with an average duration of 0.4 ms (voltage‐independent) separated by short closings with a duration of about 1.0 ms. The long‐open burst and the flickering burst appeared to reflect distinct states of a single Ca 2+ ‐activated K + channel because direct transitions between these two types of burst were often detected. We present a kinetic scheme for the gating activation pathway of a neuronal Ca 2+ ‐activated K + channel, based on these findings.