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Simultaneous Visualization of Vasopressin and Oxytocin mRNA‐ Containing Neurons in the Hypothalamus Using Non‐Radioactive in situ Hybridization Histochemistry
Author(s) -
Ichimiya Yosuke,
Emson Piers C.,
Christodoulou Chris,
Gait Michael J.,
Ruth Jerry L.
Publication year - 1989
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1111/j.1365-2826.1989.tb00081.x
Subject(s) - in situ hybridization , oxytocin , vasopressin , hypothalamus , endocrinology , medicine , neuropeptide , alkaline phosphatase , immunohistochemistry , neurophysins , biology , messenger rna , chemistry , microbiology and biotechnology , enzyme , biochemistry , receptor , gene
In situ hybridization histochemistry (ISHH) using synthetic oligodeoxynucleotide probes has been used to demonstrate the sites of expression of mRNA for vasopressin (AVP) and oxytocin (OXT) in the rat brain. ISHH was performed with two types of non‐radioactive probes, labelled with either alkaline phosphatase or 5′‐biotin. Simultaneous detection of the mRNAs for both AVP and OXT was achieved using an alkaline phosphatase substrate for the AVP probe and an anti‐biotin monoclonal (mouse) antibody for the OXT probe. These probes revealed two non‐overlapping populations of AVP and OXT neurons on the same section.