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Sensitive and Specific Radioreceptor Assay for Calcitonin Gene‐Related Peptide
Author(s) -
Wimalawansa Sunil J.
Publication year - 1989
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1111/j.1365-2826.1989.tb00071.x
Subject(s) - calcitonin gene related peptide , calcitonin , neuropeptide , peptide , medicine , endocrinology , peptide hormone , neuropeptide y receptor , receptor , vasoactive intestinal peptide , chemistry , biology , biochemistry
Calcitonin gene‐related peptide is a 37‐amino‐acid neuropeptide which has a direct action on the heart and is an extremely potent vasodilator. Immunoreactive calcitonin gene‐related peptide is present in plasma, cerebrospinal fluid and in other tissues. We have previously established the specific binding characteristics of this neuropeptide to the cerebellar membranes. This paper describes a sensitive, rapid and specific radioreceptor assay for the measurement of receptor‐active calcitonin gene‐related peptide in tissues and in plasma extracts using cerebellar membrane preparations as the binding agent. This assay should prove a valuable tool in studies of the physiology of this vasoactive neuropeptide and of its involvement in disease.