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The D3cpv Cameleon reports Ca 2+ dynamics in plant mitochondria with similar kinetics of the YC3.6 Cameleon, but with a lower sensitivity
Author(s) -
LORO G.,
RUBERTI C.,
ZOTTINI M.,
COSTA A.
Publication year - 2013
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2012.03683.x
Subject(s) - mitochondrion , organelle , arabidopsis , biophysics , biology , abiotic component , chemistry , microbiology and biotechnology , biochemistry , mutant , gene , ecology
Summary Mitochondria are key organelles involved in many aspects of plant physiology and, their ability to generate specific Ca 2+ signatures in response to abiotic and biotic stimuli has been reported as one of their roles. The recent identification of the mammalian mitochondrial Ca 2+ uniporter opens a new research area in plant biology. To study the mitochondrial Ca 2+ handling, it is essential to have a reliable probe. Here we have reported the generation of an Arabidopsis transgenic line expressing the genetically encoded probe Cameleon D3cpv targeted to mitochondria, and compared its properties with the already known Cameleon YC3.6.