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Resolution and optical sectioning in the confocal microscope
Author(s) -
WILSON T.
Publication year - 2011
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2011.03549.x
Subject(s) - optical sectioning , confocal , microscope , pinhole (optics) , optics , resolution (logic) , materials science , confocal microscopy , numerical aperture , optical microscope , wavelength , 4pi microscope , aperture (computer memory) , microscopy , fluorescence microscope , multiphoton fluorescence microscope , fluorescence , physics , computer science , scanning electron microscope , artificial intelligence , acoustics
Summary In this short review, we present a self‐contained discussion of the image formation properties of the fluorescent confocal microscope. The optical sectioning or depth discrimination property is discussed in detail and new analytic formulae are presented, which relate the optical sectioning strength to the wavelength, numerical aperture and pinhole aperture size in a particularly simple fashion.