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Correlative microscopy: a potent tool for the study of rare or unique cellular and tissue events
Author(s) -
MIRONOV A.A.,
BEZNOUSSENKO G.V.
Publication year - 2009
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2009.03222.x
Subject(s) - microscopy , electron microscope , resolution (logic) , correlative , light sheet fluorescence microscopy , super resolution microscopy , fluorescence microscope , scanning confocal electron microscopy , population , optics , fluorescence , physics , computer science , medicine , artificial intelligence , linguistics , philosophy , environmental health
Summary Biological studies have relied on two complementary microscope technologies – light (fluorescence) microscopy and electron microscopy. Light microscopy is used to study phenomena at a global scale to look for unique or rare events, and it also provides an opportunity for live imaging, whereas the forte of electron microscopy is the high resolution. Traditionally light and electron microscopy observations are carried out in different populations of cells/tissues and a ‘correlative’ inference is drawn. The advent of true correlative light‐electron microscopy has allowed high‐resolution imaging by electron microscopy of the same structure observed by light microscopy, and in advanced cases by video microscopy. Thus a rare event captured by low‐resolution imaging of a population or transient events captured by live imaging can now also be studied at high resolution by electron microscopy. Here, the potential and difficulties of this approach, along with the most impressive breakthroughs obtained by these methods, are discussed.