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Molecular cryo‐electron tomography of vitreous tissue sections: current challenges
Author(s) -
NORLÉN L.,
ÖKTEM O.,
SKOGLUND U.
Publication year - 2009
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2009.03219.x
Subject(s) - context (archaeology) , computer science , tomography , cryo electron tomography , current (fluid) , sample (material) , biomedical engineering , nanotechnology , materials science , chemistry , physics , medicine , optics , biology , chromatography , paleontology , thermodynamics
Summary Electron tomography of vitreous tissue sections (tissue TOVIS) allows the study of the three‐dimensional structure of molecular complexes in a near‐native cellular context. Its usage is, however, limited by an unfortunate combination of noisy and incomplete data, by a technically demanding sample preparation procedure, and by a disposition for specimen degradation during data collection. Here we outline some major challenges as experienced from the application of TOVIS to human skin. We further consider a number of practical measures as well as theoretical approaches for its future development.