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Controlled dehydration of a biological sample using an alternative form of environmental SEM
Author(s) -
NEDĚLA V.
Publication year - 2010
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2009.03216.x
Subject(s) - dehydration , environmental scanning electron microscope , scanning electron microscope , sample (material) , materials science , microscope , nanotechnology , thermoelectric effect , optics , chemistry , composite material , chromatography , physics , biochemistry , thermodynamics
Summary In this study a non‐conductive biological sample is observed free of charging artefacts when placed on a cooled Peltier stage in the specimen chamber of an alternative form of the environmental scanning electron microscope, equipped with a specially designed hydration system. This system was used to create dynamically changing surrounding conditions leading to controlled dehydration of the sample enabling us to visualize the topographical structure of a rat tongue in the transition region between the liquid and the gas state of water in the microscope specimen chamber.