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Light‐emitting diode flashlights as effective and inexpensive light sources for fluorescence microscopy
Author(s) -
ROBERTSON J.B.,
ZHANG Y.,
JOHNSON C.H.
Publication year - 2009
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2009.03208.x
Subject(s) - light emitting diode , fluorescence , optoelectronics , light source , microscopy , materials science , diode , fluorescence microscope , optics , excitation , blue light , optical microscope , visible spectrum , physics , scanning electron microscope , quantum mechanics
Summary Light‐emitting diodes (LEDs) are becoming more commonly used as light sources for fluorescence microscopy. We describe the adaptation of a commercially available light‐emitting diode flashlight for use as a source for fluorescence excitation. This light source is long‐lived, inexpensive and is effective for excitation in the range of 440–600 nm.