Premium
A compact STED microscope providing 3D nanoscale resolution
Author(s) -
WILDANGER D.,
MEDDA R.,
KASTRUP L.,
HELL S.W.
Publication year - 2009
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2009.03188.x
Subject(s) - sted microscopy , supercontinuum , optics , resolution (logic) , microscope , nanoscopic scale , microscopy , laser , diffraction , image resolution , cardinal point , materials science , stimulated emission , lens (geology) , physics , nanotechnology , computer science , optical fiber , artificial intelligence , photonic crystal fiber
Summary The advent of supercontinuum laser sources has enabled the implementation of compact and tunable stimulated emission depletion fluorescence microscopes for imaging far below the diffraction barrier. Here we report on an enhanced version of this approach displaying an all‐physics based resolution down to (19 ± 3) nm in the focal plane. Alternatively, this single objective lens system can be configured for 3D imaging with resolution down to 45 × 45 × 108 nm in a cell. The obtained results can be further improved by mathematical restoration algorithms. The far‐field optical nanoscale resolution is attained in a variety of biological samples featuring strong variations in the local density of features.