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Real‐time and label‐free detection of chloramphenicol residues with specific molecular interaction
Author(s) -
DONG Z.,
HUANG G.,
XU S.,
DENG C.,
ZHU J.,
CHEN S.,
YANG X.,
ZHAO S.
Publication year - 2009
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2009.03175.x
Subject(s) - surface plasmon resonance , chemistry , resonance (particle physics) , detection limit , molecular binding , analytical chemistry (journal) , biological system , molecule , materials science , nanotechnology , chromatography , organic chemistry , physics , atomic physics , nanoparticle , biology
Summary Molecular measurements with specific or non‐specific molecular interaction are usually reported by using different methods. This paper shows a method for small‐molecular label‐free measurements in real time with specific and non‐specific molecular interaction based on surface plasmon resonance. The quantitative measurement of glucose with non‐specific molecular interaction was performed simply to validate our self‐built angle‐dependent surface plasmon resonance system, which showed a good sensitivity and stability, such as that of Fourier transform infrared spectrometer. As application, we present the measurement of chloramphenicol residues with specific molecular interaction employing an indirect competitive immunoreaction by using the same surface plasmon resonance system with a limit of detection about 0.5 ng mL −1 , which shows a potential application for food safety.