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Assessment of acridine orange and SYTO 16 for in vivo imaging of the peritoneal tissues in mice
Author(s) -
UDOVICH J.A.,
BESSELSEN D.G.,
GMITRO A.F.
Publication year - 2009
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2009.03153.x
Subject(s) - acridine orange , in vivo , confocal , acridine , saline , endoscope , pathology , confidence interval , biology , chemistry , staining , medicine , surgery , optics , physics , microbiology and biotechnology , genetics
Summary The effect of peritoneal injection of acridine orange and SYTO 16 in mice was investigated. Images of peritoneal tissues stained with these dyes and obtained through a confocal micro‐endoscope are presented. Seventy‐five Balb/c mice were split into five groups and given peritoneal injections of dye or saline. The proportions of negative outcomes in each group were compared using confidence intervals and the Fisher's exact statistical test. A statistically significant increase in adverse events due to dye injection was not observed. These data provide an initial investigation into the safety of acridine orange and SYTO 16 for in vivo imaging.