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Self‐calibration method for vision‐guided cell micromanipulation systems
Author(s) -
ZHANG Y.L.,
HAN M.L.,
SHEE C.Y.,
CHIA T.F.,
ANG W.T.
Publication year - 2009
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2009.03123.x
Subject(s) - micromanipulator , calibration , computer vision , computer science , artificial intelligence , position (finance) , matrix (chemical analysis) , standard deviation , actuator , control theory (sociology) , mathematics , materials science , statistics , finance , economics , composite material , control (management)
Summary A new self‐calibration method for a piezoelectric actuator‐based vision‐guided cell micromanipulation system is proposed. This method consolidates all the system parameters' uncertainties into a matrix instead of classifying into intrinsic and extrinsic. The position difference of the micromanipulator tip in the image plane between the measured and estimated output which is based on estimations of relevant parameters is assumed to be caused by the matrix. This matrix is estimated by means of collecting several pairs of known input and the corresponding output differences. Matrix standard deviation and gray‐value based matching are applied to identify the output differences. Biological contamination is reduced since a calibration template is not required. This self‐calibration is particularly suitable for cell micromanipulation systems where the micromanipulator is frequently dismounted and mounted.