Premium
Stroboscopic near‐field imaging for the analysis of contraction of muscle cells
Author(s) -
MORIGUCHI C.,
EGAMI C.,
KAWATA Y.,
TERAKAWA S.,
TSUCHIMORI M.,
WATANABE O.
Publication year - 2009
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2009.03117.x
Subject(s) - stroboscope , optics , optical microscope , materials science , microscope , wavelength , microscopy , bright field microscopy , high resolution , resolution (logic) , biomedical engineering , scanning electron microscope , physics , computer science , remote sensing , medicine , artificial intelligence , geology
Summary We have developed a stroboscopic near‐field optical microscope for observation of biological specimens and observed glycerinated muscles before and after muscle contraction with the developed system. In the system, the optical field distribution localized near the specimen is recorded as the surface topographic distribution of a photosensitive film surface. Our system is very useful for observing living biological specimens with high resolutions, because it is possible to get stroboscopic image by using a photosensitive film as detecting optical distributions instead of a scanning of probes. We have succeeded in observing inner structures of muscle cells with sub‐wavelength resolution and achieved higher contrast than an ordinary optical microscope.