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Early effects of gamma rays and protons on human melanoma cell viability and morphology
Author(s) -
TODOROVIĆ D.,
PETROVIĆ I.,
TODOROVIĆ M.,
CUTTONE G.,
RISTIĆFIRA A.
Publication year - 2008
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2008.02151.x
Subject(s) - irradiation , proton , gamma ray , ionizing radiation , viability assay , bragg peak , radiochemistry , morphology (biology) , cell , chemistry , biophysics , cell growth , radiation , physics , biology , nuclear physics , biochemistry , genetics
Summary The effects of irradiation with gamma rays and protons on HTB140 human melanoma cell morphology and viability were analyzed. Exponentially growing cells were irradiated close to the Bragg peak maximum of the 62‐MeV proton beam, as well as with 60 Co gamma rays, with doses ranging from 8 to 24 Gy. The overall cell morphology was unchanged 6 and 48 h after gamma irradiation, also showing a relatively weak cell‐inactivation level. After exposure to proton beam, considerable changes in cell morphology followed by stronger cell inactivation were achieved. Proliferation capacity of irradiated cells significantly decreased in both experimental set‐ups. Higher ionization level of protons with respect to gamma rays, representing the main physical difference between these two types of radiation, was also revealed on the cell membrane level through larger pro‐apoptotic capacity of protons.