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Immersion oil for high‐resolution live‐cell imaging at 37°C: optical and physical characteristics
Author(s) -
OOMEN L.C.J.M.,
SACHER R.,
BROCKS H.H.J.,
ZWIER J.M.,
BRAKENHOFF G.J.,
JALINK K.
Publication year - 2008
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2008.02106.x
Subject(s) - oil immersion , immersion (mathematics) , materials science , resolution (logic) , high resolution , optics , chemistry , computer science , physics , remote sensing , artificial intelligence , composite material , geology , mathematics , pure mathematics
Summary The use of normal immersion oil, developed for 23°C, at 37°C greatly compromises both axial resolution and signal intensity. We developed and characterized an immersion oil for optimal performance in live‐cell imaging at 37°C. We quantify the improvements in resolution and intensity obtained when using the new oil instead of its standard 23°C counterparts.