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Compression and crevasses in vitreous sections under different cutting conditions
Author(s) -
HAN H.M.,
ZUBER B.,
DUBOCHET J.
Publication year - 2008
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2008.01972.x
Subject(s) - compression (physics) , materials science , optics , composite material , physics
Summary Compression and crevasses are common cutting artefacts in cryo‐ultramicrotomy of vitreous sections. They can be reduced or suppressed under optimal cutting conditions. In the present study, compression and thickness were measured for different cutting speeds and knife angles. It was found that compression decreased with feed and that crevasses appeared only above a certain thickness. The optimal feed for vitreous sections was between 50 and 80 nm. The thickness, calculated by two independent methods, was quantitatively related to feed and compression.