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Cell wall investigations utilizing tip‐enhanced Raman scattering
Author(s) -
BUDICH C.,
NEUGEBAUER U.,
POPP J.,
DECKERT V.
Publication year - 2008
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2008.01939.x
Subject(s) - raman scattering , scattering , signal (programming language) , optics , raman spectroscopy , field (mathematics) , transmission (telecommunications) , staphylococcus epidermidis , resolution (logic) , emphasis (telecommunications) , materials science , nanotechnology , computer science , physics , geology , artificial intelligence , telecommunications , bacteria , paleontology , mathematics , pure mathematics , programming language , staphylococcus aureus
Summary Tip‐enhanced Raman scattering is used to investigate the surface structure of the cell wall of Staphylococcus epidermidis with high lateral resolution and chemical specificity. For most biological samples, the transmission tip‐enhanced Raman scattering set‐up is ideally suited because it allows the specimen to be kept under specific environmental conditions, whereas it most efficiently collects the signal created at the field‐enhancing probe. Special emphasis is given here to the parameters required to reproducibly set up the instrument, such that field‐enhancement factors can be estimated properly. Also the importance of control experiments to avoid misinterpretation of signals will be emphasized by an example.