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Confocal microscopy study of the distribution, content and activity of mitochondria during Paracentrotus lividus development
Author(s) -
MORICI GIOVANNI,
AGNELLO MARIA,
SPAGNOLO FILIPPO,
ROCCHERI MARIA CARMELA,
LIEGRO CARLO MARIA DI,
RINALDI ANNA MARIA
Publication year - 2007
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2007.01860.x
Subject(s) - paracentrotus lividus , sea urchin , organelle , confocal microscopy , mitochondrion , microbiology and biotechnology , embryo , confocal , fluorescence microscope , oxidative phosphorylation , biophysics , biology , respiration , chemistry , fluorescence , biochemistry , anatomy , physics , geometry , mathematics , quantum mechanics
Summary In the present paper we applied confocal microscopy and fluorescence technologies for studying the distribution and the oxidative activity of sea urchin ( Paracentrotus lividus ) mitochondria during development, by in vivo incubating eggs and embryos with cell‐permeant MitoTracker probes. We calculated, by a mathematical model, the intensity values, the variations of intensity, and the variation index of incorporated fluorochromes. Data demonstrate that mitochondrial mass does not change during development, whereas mitochondrial respiration increases. In addition, starting from 16 blastomeres stage, some regions of the embryo contain organelles more active in oxygen consumption.