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Specimen‐induced distortions in light microscopy
Author(s) -
SCHWERTNER M.,
BOOTH M. J.,
WILSON T.
Publication year - 2007
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2007.01827.x
Subject(s) - optics , wavefront , microscopy , biological specimen , numerical aperture , materials science , image quality , aperture (computer memory) , resolution (logic) , spherical aberration , image resolution , interferometry , signal (programming language) , transmission (telecommunications) , physics , image (mathematics) , computer science , wavelength , computer vision , lens (geology) , artificial intelligence , acoustics , programming language , telecommunications
Summary Specimen‐induced aberrations affect the imaging properties in optical 3D microscopy, especially when high numerical aperture lenses are used. Studies on aberrations are often properly concerned with the degradation of image quality such as compromised resolution or reduced signal intensity. Apart from these, aberration effects can also introduce geometric image distortions. The effects, discussed here are particularly strong when thick biological specimens are investigated. Using a high numerical aperture interferometer, we measured wavefront aberrations in transmission mode and quantified geometric distortions associated with specimen‐induced aberrations. This assessment for a range of biological specimens allows estimation of the accuracy of spatial measurements. The results show that high‐resolution spatial measurements can be significantly compromised by specimen‐induced aberrations.