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Small‐scale in vivo imaging of soft tissues by radioscopy using single X‐ray photon counting
Author(s) -
HANUS ROBERT,
DAMMER JIŘÍ,
HOLÝ TOMÁŠ,
JAKŮBEK JAN,
POSPÍŠIL STANISLAV,
TYKVA RICHARD
Publication year - 2007
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2007.01778.x
Subject(s) - tomographic reconstruction , tomography , visualization , biomedical engineering , image resolution , photon counting , preclinical imaging , soft tissue , scale (ratio) , computer science , medical physics , in vivo , materials science , optics , computer vision , radiology , photon , medicine , physics , artificial intelligence , biology , microbiology and biotechnology , quantum mechanics
Summary A method has been developed for routine laboratory visualisation of small‐scale soft tissue by means of transmission X‐ray radioscopy and tomography. Using termites as models, imaging quality with a spatial resolution of about 3 μm was achieved and 3D tomographic reconstruction was demonstrated. A termite worker individual was visualized before and after its metamorphosis towards the soldier caste. The developed methodology represents a non‐invasive and real‐time way of acquiring 3D anatomic data with a high contrast so that it is a promising candidate to become a tool for routine investigations in life sciences.