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Modelling the application of adaptive optics to wide‐field microscope live imaging
Author(s) -
KAM ZVI,
KNER PETER,
AGARD DAVID,
SEDAT JOHN W.
Publication year - 2007
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2007.01751.x
Subject(s) - microscope , optics , lens (geology) , adaptive optics , fluorescence microscope , microscopy , computer science , diffraction , field (mathematics) , image quality , fluorescence lifetime imaging microscopy , physics , fluorescence , computer vision , image (mathematics) , mathematics , pure mathematics
Summary Wide‐field fluorescence microscopy is an essential tool in modern cell biology. Unfortunately the image quality of fluorescence microscopes is often significantly degraded due to aberrations that occur under normal imaging conditions. In this article, we examine the use of adaptive optics technology to dynamically correct these problems to achieve close to ideal diffraction limited performance. Simultaneously, this technology also allows ultra‐rapid focusing without having to move either the stage or the objective lens. We perform optical simulations to demonstrate the degree of correction that can be achieved.