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Photo‐physical properties of Ca 2+ ‐indicator dyes suitable for two‐photon fluorescence‐lifetime recordings
Author(s) -
WILMS CHRISTIAN D.,
EILERS JENS
Publication year - 2007
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2007.01746.x
Subject(s) - fluorescence , chemistry , impurity , calcein , calcium , analytical chemistry (journal) , photon , attenuation , photochemistry , optics , physics , chromatography , organic chemistry
Summary We analyzed the suitability of various Ca 2+ ‐indicator dyes for quantitative two‐photon fluorescence‐lifetime imaging. Although fura‐2, fluo‐3, BTC and calcein did not show useful Ca 2+ ‐dependent lifetime changes, calcium orange, calcium green‐1, oregon green‐2 and ‐5N, as well as magnesium green allowed to quantify the Ca 2+ ‐free and Ca 2+ ‐bound dye fractions by a double‐exponential lifetime analysis. For the latter dyes, we derived calibration formalisms that correct for lifetime distortions by dye impurities and Ca 2+ ‐dependent extinction coefficients.