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In situ atomic force microscopy and image analysis of dentine submitted to acid etching
Author(s) -
PACIORNIK S.,
DEDEUS G.,
REIS C. M.,
PINHO MAURICIO M. H.,
PRIOLI R.
Publication year - 2007
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2007.01738.x
Subject(s) - etching (microfabrication) , atomic force microscopy , in situ , microscopy , materials science , acid etching , isotropic etching , chemistry , analytical chemistry (journal) , nanotechnology , composite material , optics , chromatography , physics , organic chemistry , layer (electronics)
Summary The effect of acid etching on root dentine was analyzed with Atomic Force Microscopy using a liquid cell that allowed image acquisition while the sample was kept in contact with the etching solution. The time evolution of the etching process was evaluated both qualitatively and quantitatively. Image sequences against etching time were obtained showing a clear trend of surface cleaning and opening of dentine tubules. Image analysis methods were employed to quantify the changes on the dentine surface, through the detection and morphological measurement of dentine tubules over time. The measurements indicated an overall increase in the diameter of the tubules against time, with a saturation value between 3.5 and 5.5 μm reached after approximately 300 seconds.