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Integration of a scanning ion conductance microscope into phase contrast optics and its application to the quantification of morphological parameters of selected cells
Author(s) -
MANN S. A.,
MEYER J. W.,
DIETZEL I. D.
Publication year - 2006
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2006.01693.x
Subject(s) - phase contrast microscopy , microscope , scanning electron microscope , materials science , conductance , optics , phase (matter) , scanning ion conductance microscopy , contrast (vision) , optical microscope , biomedical engineering , chemistry , scanning confocal electron microscopy , physics , medicine , organic chemistry , condensed matter physics
Summary We have previously described a pulse‐mode scanning ion conductance microscope to investigate membrane surfaces and volume changes of individual cells in culture. We have now developed a miniaturized scanning headstage that enables us to select individual cells for recording under phase contrast optics, considerably improving the selection of individual cells for scanning as well as the positioning of the scanning frames with respect to the position of the cell somata. We show an application in which surfaces and volumes of somata and processes of cultured cells from the central nervous system were quantified separately.