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Epifluorescence, confocal and total internal reflection microscopy for single‐molecule experiments: a quantitative comparison
Author(s) -
LANG E.,
BAIER J.,
KÖHLER J.
Publication year - 2006
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2006.01579.x
Subject(s) - confocal , total internal reflection fluorescence microscope , confocal microscopy , microscopy , fluorescence , total internal reflection , microscope , fluorescence microscope , reflection (computer programming) , optics , molecule , optical microscope , chemistry , materials science , biophysics , physics , biology , scanning electron microscope , computer science , programming language , organic chemistry
Summary Epifluorescence, confocal and total internal reflection microscopy are the most widely used techniques for optical single‐molecule experiments. Employing these methods, we recorded the emission intensity of the same single molecule as a function of the excitation rate under otherwise identical experimental conditions. Evaluation of these data provides a quantitative comparison of the signal‐to‐background ratios that can be achieved for the three microscopic techniques.