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Laser autofocusing system for high‐resolution cell biological imaging
Author(s) -
LIRON Y.,
PARAN Y.,
ZATORSKY N. G.,
GEIGER B.,
KAM Z.
Publication year - 2006
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2006.01550.x
Subject(s) - focus (optics) , microscopy , resolution (logic) , autofocus , laser , microscope , computer science , biological specimen , high resolution , optics , computer vision , artificial intelligence , physics , remote sensing , geology
Summary Automated acquisition of high resolution, light microscope images of cells is becoming a common requirement in modern proteomic and cellomic research. A prerequisite for such microscopy is fine focus tuning, commonly optimized by multiple exposures, followed by image sharpness analysis. We describe here an extremely fast and accurate laser autofocusing system with distinct advantages for large‐scale cell‐based screening.