Premium
Core‐shell CdS/Cd(OH) 2 quantum dots: synthesis and bioconjugation to target red cells antigens
Author(s) -
ALBUQUERQUE DE FARIAS P. M.,
SAEGESSER SANTOS B.,
DUARTE DE MENEZES F.,
DE CARVALHO FERREIRA R.,
DE LOURDES BARJASCASTRO M.,
CASTRO V.,
MOURA LIMA P. R.,
FONTES A.,
L. CESAR C.
Publication year - 2005
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2005.01501.x
Subject(s) - bioconjugation , quantum dot , luminescence , confocal , chemistry , hydroxide , nanocrystal , colloid , monoclonal antibody , labelling , glutaraldehyde , nanotechnology , materials science , antibody , optoelectronics , optics , inorganic chemistry , biochemistry , biology , chromatography , physics , immunology
Summary We report a new and efficient methodology of labelling red blood cells, in order to investigate the expression of anti‐A antigen, employing luminescent semiconductor nanocrystals. Highly luminescent and stable core‐shell cadmium sulphide/cadmium hydroxide [CdS/CdS(OH) 2 ] colloidal particles were obtained in the nanometre size range. The surface of these particles was characterized by using a monoclonal anti‐A antibody via a one‐step glutaraldehyde cross‐linking procedure, followed by conjugation of the particles to red cells of blood groups A + , and O + . Laser scanning confocal microscopy images indicated that after conjugation for 30 min, A + and erythrocytes presented different patterns of dual bright emission whereas the O + group cells showed no emission. We suggest that this labelling procedure may be applied as a quantitative tool to investigate the distribution and expression of alloantigen in red blood cells.