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Immobilization and condensation of DNA with 3‐aminopropyltriethoxysilane studied by atomic force microscopy
Author(s) -
LIU Z.,
LI Z.,
ZHOU H.,
WEI G.,
SONG Y.,
WANG L.
Publication year - 2005
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2005.01482.x
Subject(s) - mica , atomic force microscopy , condensation , evaporation , substrate (aquarium) , materials science , rod , dna , chemical engineering , nanotechnology , microscopy , chemistry , analytical chemistry (journal) , composite material , optics , chromatography , medicine , biochemistry , oceanography , physics , alternative medicine , pathology , geology , engineering , thermodynamics
Summary We used different methods to modify a mica surface with 3‐aminopropyltriethoxysilane (APTES), and then used it as substrate to immobilize DNA for atomic force microscopy (AFM) observation. The evaporation method and solution modifying method were investigated and evaluated. The solution modifying method was found to be relatively simple and effective. Using an APTES solution‐modified mica surface, DNA immobilization appeared more reproducible and it could be imaged in liquid. The mixed solution of APTES and DNA was dropped directly onto the mica surface for AFM imaging. We found that DNA can condense in APTES water solutions. Toroids, rods and intermediate structures of condensation were captured by AFM.