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SNOM and AFM microscopy techniques to study the effect of non‐ionizing radiation on the morphological and biochemical properties of human keratinocytes cell line (HaCaT)
Author(s) -
Rieti S.,
Manni V.,
Lisi A.,
Giuliani L.,
Sacco D.,
D’Emilia E.,
Cricenti A.,
Generosi R.,
Luce M.,
Grimaldi S.
Publication year - 2004
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2004.01279.x
Subject(s) - hacat , microscopy , atomic force microscopy , adhesion , biophysics , cell adhesion , immunofluorescence , near field scanning optical microscope , confocal microscopy , materials science , integrin , chemistry , microbiology and biotechnology , cell , optics , nanotechnology , optical microscope , scanning electron microscope , biology , antibody , in vitro , immunology , physics , biochemistry , composite material
Summary In this study we have employed atomic force microscopy (AFM) and scanning near‐field optical microscopy (SNOM) techniques to study the effect of the interaction between human keratinocytes (HaCaT) and electromagnetic fields at low frequency. HaCaT cells were exposed to a sinusoidal magnetic field at a density of 50 Hz, 1 mT. AFM analysis revealed modification in shape and morphology in exposed cells with an increase in the areas of adhesion between cells. This latter finding was confirmed by SNOM indirect immunofluorescence analysis performed with a fluorescent antibody against the adhesion marker β4 integrin, which revealed an increase of β4 integrin segregation in the cell membrane of 50‐Hz exposed cells, suggesting that a higher percentage of these cells shows a modified pattern of this adhesion marker.