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Oriented immobilization of Pseudomonas putida putidaredoxin at a gold (111)–buffer interface: a real time scanning tunnelling microscopy study
Author(s) -
Mukhopadhyay R.,
Lo K. K.,
Wong L. L.,
Hill H. A. O.
Publication year - 2004
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2004.01246.x
Subject(s) - pseudomonas putida , scanning tunneling microscope , microscopy , quantum tunnelling , scanning electron microscope , materials science , nanotechnology , interface (matter) , buffer (optical fiber) , chemistry , analytical chemistry (journal) , chemical engineering , optics , optoelectronics , adsorption , computer science , chromatography , physics , engineering , composite material , organic chemistry , enzyme , telecommunications , gibbs isotherm
Summary A scanning tunnelling microscopy study of adsorption of wild‐type Pseudomonas putida putidaredoxin at a gold (111)–buffer interface has been made in real time . Reversible adsorption has been observed reflecting weak interaction of the wild‐type protein with a gold (111) electrode. A genetically engineered mutant, C73S‐D58C, which contains a surface thiol, has been used for ‘immobilization’ and ‘orientated adsorption’ on the gold surface. The implication of such orientated immobilization in development of a bio‐electrode surface has been predicted.