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Fluorescence in situ hybridization on human metaphase chromosomes detected by near‐field scanning optical microscopy
Author(s) -
MOERS M. H. P.,
KALLE W. H. J.,
RUITER A. G. T.,
WIEGANT J. C. A. G.,
RAAP A. K.,
GREVE J.,
GROOTH B. G.,
HULST N. F.
Publication year - 1996
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1996.tb04795.x
Subject(s) - metaphase , fluorescence in situ hybridization , in situ , microscopy , hybridization probe , fluorescence , fluorescence microscope , microbiology and biotechnology , resolution (logic) , optics , biology , chromosome , in situ hybridization , chemistry , dna , genetics , physics , gene , gene expression , organic chemistry , artificial intelligence , computer science
Summary Fluorescence in situ hybridization on human metaphase chromosomes is detected by near‐field scanning optical microscopy. This combination of cytochemical and scanning probe techniques enables the localization and identification of several fluorescently labelled genomic DNA fragments on a single chromosome with an unprecedented resolution. Three nucleic acid probes are used: pUC1. 77. p1–79 and the plasmid probe α‐spectrin. The hybridization signals are very well resolved in the near‐field fluorescence images, while the exact location of the probes can be correlated accurately with the chromosome topography as afforded by the shear force image.