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Direct‐view microscopy: optical sectioning strength for finite‐sized, multiple‐pinhole arrays
Author(s) -
McCABE E. M.,
FEWER D. T.,
OTTEWILL A. C.,
HEWLETT S. J.,
HEGARTY J.
Publication year - 1996
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1996.tb00004.x
Subject(s) - optical sectioning , pinhole (optics) , optics , optical microscope , microscopy , materials science , microscope , aperture (computer memory) , hexagonal crystal system , interleaving , fluorescence microscope , spiral (railway) , light sheet fluorescence microscopy , scanning confocal electron microscopy , physics , fluorescence , computer science , scanning electron microscope , chemistry , mathematics , acoustics , mathematical analysis , crystallography , operating system
Summary Direct‐view microscopes use multiple‐aperture arrays in the source and detector planes. We develop a theory for brightfleld and fluorescence direct‐view microscopy which allows us to determine the optical sectioning strength for finite‐sized, multiple‐pinhole arrays with an arbitrary distribution of apertures. We specialize to the cases of square, hexagonal and interleaving Archimedean spiral arrays and consider the implications of the array configuration on both the optical sectioning strength and the light budget.