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Easily prepared holey films for use in cryo‐electron microscopy
Author(s) -
JAHN W.
Publication year - 1995
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1995.tb03649.x
Subject(s) - mica , dichloromethane , electron microscope , materials science , glycerol , chemical engineering , emulsion , optical microscope , photographic emulsion , electron , microscopy , scanning electron microscope , crystallography , chemistry , optics , organic chemistry , composite material , solvent , silver halide , physics , engineering , quantum mechanics
Summary Holey films or nets may be produced from an emulsion of glycerol/water in a solution of a mixture of Formvar® and Triafol® in dichloromethane, spread on mica. After being floated off the mica support the films may be treated with ethyl acetate to transform ‘pseudoholes' into holes or nets.