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On the extinction coefficient in confocal polarization microscopy
Author(s) -
WILSON T.,
JUŠKAITIS R.
Publication year - 1995
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1995.tb03636.x
Subject(s) - microscope , confocal , optics , confocal microscopy , polarization (electrochemistry) , microscopy , polarized light microscopy , materials science , physics , chemistry
Summary The extinction coefficient in conventional polarized light microscopes is non‐zero, even with perfect polars, solely because of the image formation process in these instruments. The imaging in confocal microscopes is different from conventional instruments and it is shown that in this case an infinite extinction coefficient results in the ideal case whereas a finite value is always to be expected with conventional instruments. Images of the same microelectronic specimen taken in both conventional and confocal polarized light microscopes are compared.