Premium
Comparison of three‐dimensional imaging properties between two‐photon and single‐photon fluorescence microscopy
Author(s) -
GU MIN,
SHEPPARD C. J. R.
Publication year - 1995
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1995.tb03543.x
Subject(s) - confocal , optics , microscopy , point spread function , confocal microscopy , fluorescence , fluorescence lifetime imaging microscopy , two photon excitation microscopy , resolution (logic) , photon , optical sectioning , fluorescence microscope , materials science , optical transfer function , physics , transverse plane , photoactivated localization microscopy , light sheet fluorescence microscopy , super resolution microscopy , biology , anatomy , computer science , artificial intelligence
SUMMARY The imaging performance in single‐photon (1‐p) and two‐photon (2‐p) fluorescence microscopy is described. Both confocal and conventional systems are compared in terms of the three‐dimensional (3‐D) point spread function and the 3‐D optical transfer function. Images of fluorescent sharp edges and layers are modelled, giving resolution in transverse and axial directions. A comparison of the imaging properties is also given for a 4Pi confocal system. Confocal 2‐p 4Pi fluorescence microscopy gives the best axial resolution in the sense that its 3‐D optical transfer function has the strongest response along the axial direction.