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A simple method for overcoming some problems when observing thick reflective biological samples with a confocal scanning laser microscope
Author(s) -
RUMIO C.,
MORINI M.,
MIANI A.,
BARAJON I.,
CASTANO P.
Publication year - 1995
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1995.tb03536.x
Subject(s) - confocal , microscope , materials science , optics , opacity , laser , laser microscopy , laser scanning , optical microscope , laser beams , confocal laser scanning microscopy , biomedical engineering , scanning electron microscope , composite material , physics , medicine
Summary A simple device is described, which allows the range of depth of scanning to be reduced when observing thick reflecting biological samples with a confocal scanning laser microscope (CSLM). Thick histological sections of human skin and rat brain stem were mounted between two coverslips (‘sandwich’ style) and the optical tomography was performed from both sides by turning the ‘sandwich’ upside‐down. The samples were impregnated using standard Golgi–Cox, ‘rapid Golgi’ or other silver methods. The ability to turn the ‘sandwich’ upside‐down is particularly useful when the reflective structure inspected is deep inside the section, i.e. near the lower surface of the specimen, or when it is opaque to the laser beam or excessively reflective.