Premium
A simple method for processing individual oocytes and embryos for electron microscopy
Author(s) -
NOGUÉS C.,
MARTÍ M.,
BOADA M.,
PONSÁ M.
Publication year - 1994
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1994.tb04324.x
Subject(s) - electron microscope , simple (philosophy) , embryo , microbiology and biotechnology , microscopy , computer science , biology , computational biology , physics , optics , philosophy , epistemology
Summary A simple method for handling individual specimens that must be processed either for scanning or transmission electron microscopy studies is described. For scanning microscope processing, dehydration is carried out with samples enclosed in small cages made from TAAB capsules in which top and bottom are substituted by plankton nets, and for transmission electron microscopy, samples are pre‐embedded in agarose. This procedure significantly reduces mouth pipetting, dissecting microscope observations, is less labour intensive and, most importantly, reduces sample loss.