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Continuous wave excitation two‐photon fluorescence microscopy
Author(s) -
HÄNNINEN P. E.,
SOINI E.,
HELL S. W.
Publication year - 1994
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1994.tb03518.x
Subject(s) - two photon excitation microscopy , microscopy , fluorescence , excitation , fluorescence microscope , multiphoton fluorescence microscope , optics , materials science , photon , physics , nuclear magnetic resonance , chemistry , quantum mechanics
Two‐photon excitation fluorescence imaging is feasible with continuous wave lasers. Images of biological specimens are obtained by employing photon counting in conjunction with an increased recording time. The approach allows two‐photon three‐dimensional imaging of fluorescently labelled specimens with inexpensive lasers.