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Relocation accuracy on HOME computerized microscopes
Author(s) -
TUCKER J. H.,
DYE R.,
SPREY J.,
SOWTER C.,
GRAY E.,
BRUGAL G.
Publication year - 1994
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1994.tb03501.x
Subject(s) - microscope , relocation , computer science , optical microscope , standard deviation , microscopy , computer vision , artificial intelligence , computer graphics (images) , materials science , optics , physics , mathematics , statistics , scanning electron microscope , programming language
Summary A major practical advantage of the HOME (highly optimized microscope environment) computerized microscope is the facility for relocating cells or other microscopic objects. Features can be marked directly on the microscope image using a mouse‐driven cursor, and an interactive finder can then be used to relocate the marked features. Tests on a prototype HOME microscope have shown that positions can be relocated with an accuracy of standard deviation (SD) < 7 μm. The marked features could also be relocated on a second HOME microscope, although with somewhat reduced accuracy (standard deviations of < 17 μm). The system provides a very user‐friendly environment for tasks requiring relocation of microscopic objects.