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Rapid assay for pathogenic salmonella organisms by immunofluorescence flow cytometry
Author(s) -
PINDER A. C.,
MCCLELLAND R. G.
Publication year - 1994
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1994.tb03495.x
Subject(s) - flow cytometry , salmonella , monoclonal antibody , escherichia coli , detection limit , immunofluorescence , microbiology and biotechnology , serotype , biology , cytometry , chemistry , enterobacteriaceae , antibody , chromatography , bacteria , biochemistry , immunology , genetics , gene
Summary Multi‐parameter flow cytometry was investigated for the rapid detection of specific serotypes of salmonellas ( S. typhimurium and S. montevideo ) labelled with fluorescent monoclonal antibodies, both in pure culture and in a typical food matrix (full‐fat milk). In all cases, the method was accurate to levels of below 10 4 target cells per ml for a total assay time of about 30 min. After 6 h non‐selective enrichment in the presence of a 10 000‐fold excess of competing micro‐organisms ( Escherichia coli ) the corresponding detection limit was about 20 cells ml −1 . These results suggest that flow cytometry has significant potential for the detection of pathogenic micro‐organisms in the food industry.