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ESI in situ analyses of extrachromosomal RNP granules
Author(s) -
ABOLHASSANIDADRAS S.,
VÁZQUEZNIN G. H.,
ECHEVERRÍA O. M.,
ROUELLEROSSIER V. BOUTINARD,
FAKAN S.
Publication year - 1994
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1994.tb03470.x
Subject(s) - ribonucleoprotein , nucleoplasm , staining , in situ , granule (geology) , transmission electron microscopy , electron microscope , chemistry , biophysics , biology , materials science , rna , physics , cytoplasm , nanotechnology , optics , biochemistry , nucleolus , paleontology , genetics , gene , organic chemistry
Summary Observation of unstained ultrathin sections of salivary gland cells of Chironomus thummi and C. tentans , by means of electron spectroscopic imaging (ESI), has revealed that phosphorus is distributed in two types of granular structures in the nucleoplasm of these cells. In addition to a specific type of premessenger ribonucleoprotein (RNP) particle, known as the Balbiani ring (Br) granule, ESI revealed a new type of phosphorus‐rich small granular component. Examination of unstained sections by energy‐filtering transmission electron microscopy (EFTEM) offers the opportunity of obtaining the signal from the specimen alone, thus avoiding the possible contributions of heavy metals present in any staining product.