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Demonstration of aluminium in polyphosphate of Laccaria amethystea (Bolt. ex Hooker) Murr. by means of electron energy‐loss spectroscopy
Author(s) -
KOTTKE I.,
MARTIN F.
Publication year - 1994
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1994.tb03469.x
Subject(s) - aluminium , mycelium , polyphosphate , ectomycorrhiza , electron energy loss spectroscopy , paxillus involutus , fungus , spectroscopy , chemistry , materials science , botany , biology , mycorrhiza , metallurgy , physics , symbiosis , nanotechnology , biochemistry , genetics , phosphate , quantum mechanics , transmission electron microscopy , bacteria
Summary Toxic aluminium species in acidified soil damage the rootlets of trees. The symbiotic association with ectomycorrhizal fungi may, however, protect the rootlets by sequestration of aluminium in polyphosphates. Electron energy‐loss imaging and spectroscopy was used to identify the relative amount of aluminium in the polyphosphate of the mycelium of the ectomycorrhizal fungus Laccaria amethystea . A three‐window method at the L 2,3 edge yielded results which were in agreement with the spectra obtained at the aluminium K edge. The aluminium net distribution images were recorded at 85 eV, the backgrounds at 70 eV and 60 eV, setting the energy selecting slit to 5 eV and the beam current to 15 μA. The method can be used to study the sequestration of aluminium in mycelia of ectomycorrhizal fungi under different stress situations.