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Severe shrinkage of Microfil during tissue clearing with the Spalteholz technique
Author(s) -
MØLLER J.,
ROBERTSEN K.,
HANSEN E. S.
Publication year - 1994
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1994.tb03457.x
Subject(s) - shrinkage , clearance , clearing , silicone , materials science , chemistry , composite material , medicine , finance , economics , urology
Summary The effect of two commonly used tissue clearing methods on the morphology of vascular casts formed by Microfil, a silicone rubber injection compound, was investigated. Microfil undergoes extreme shrinkage when the casted tissue is cleared by the alchohol‐methyl salicylate clearing technique. No shrinkage is observed when the alternative glycerin clearing method is used. The alcohol‐methyl salicylate clearing technique should be avoided in studies employing Microfil.