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Method for the study of the three‐dimensional orientation of the nuclei of myocardial cells in fetal human heart by means of confocal scanning laser microscopy
Author(s) -
USSON Y.,
PARAZZA F.,
JOUK P.S.,
MICHALOWICZ G.
Publication year - 1994
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1994.tb03454.x
Subject(s) - confocal laser scanning microscopy , laser microscopy , confocal , confocal microscopy , microscopy , orientation (vector space) , materials science , fetal heart , fetus , chemistry , biomedical engineering , biophysics , optics , pathology , medicine , physics , biology , mathematics , pregnancy , geometry , genetics
Summary A series of three‐dimensional image analysis tools are used to measure the three‐dimensional orientation of nuclei of myocardial cells. Confocal scanning laser microscopy makes it possible to acquire series of sections up to 100 μm inside thick tissue sections. A mean orientation vector of unit length is calculated for each segmented nucleus. The global orientation statistics are obtained by calculating the vectorial sum of the nuclear unit vectors. The final orientation is expressed by a mean azimuth angle, an elevation angle and a measure of the angular homogeneity. The method is illustrated for two different regions of the myocardium (interventricular septum and papillary muscle) of a normal human fetal heart. This quantitative method will be used to assess and calibrate the information provided by polarized light microscopy.